FBL Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: FBL Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-15-018
• Supplier Catalog Number: 15-018
• Alternative Catalog Number: PRS-15-018-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).
• Conjugation: Unconjugated
• Alternative Names: FBL, FIB, FLRN, RNU3IP1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 37kDa
• NCBI: 2091
• UniProt: P22087
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Images

Western blot analysis of extracts of various cell lines, using FBL antibody (15-018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse liver using FBL antibody (15-018) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-251MG cells using FBL antibody (15-018) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FBL antibody (15-018). Western blot was performed from the immunoprecipitate using FBL antibody (15-018) at a dilition of 1:1000.