p27 KIP 1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: p27 KIP 1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-16-151
• Hersteller Artikelnummer: 16-151
• Alternativnummer: PRS-16-151-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p27 KIP 1 (NP_004055.1).
• Konjugation: Unconjugated
• Alternative Synonym: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 30kDa
• NCBI: 1027
• UniProt: P46527
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using p27 KIP 1 antibody (16-151) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat testis using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).