p27 KIP 1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: p27 KIP 1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-151
• Supplier Catalog Number: 16-151
• Alternative Catalog Number: PRS-16-151-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p27 KIP 1 (NP_004055.1).
• Conjugation: Unconjugated
• Alternative Names: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 30kDa
• NCBI: 1027
• UniProt: P46527
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using p27 KIP 1 antibody (16-151) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat testis using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using p27 KIP 1 antibody (16-151) at dilution of 1:100 (40x lens).