HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-16-418
• Hersteller Artikelnummer: 16-418
• Alternativnummer: PRS-16-418-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2).
• Konjugation: Unconjugated
• Alternative Synonym: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 37kDa
• NCBI: 1994
• UniProt: Q15717
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (16-418) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (16-418) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse testis using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of MCF-7 cells using HuR / ELAVL1 antibody (16-418). Blue: DAPI for nuclear staining.