HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-418
• Supplier Catalog Number: 16-418
• Alternative Catalog Number: PRS-16-418-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2).
• Conjugation: Unconjugated
• Alternative Names: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 37kDa
• NCBI: 1994
• UniProt: Q15717
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (16-418) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (16-418) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse testis using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR / ELAVL1 Antibody (16-418) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of MCF-7 cells using HuR / ELAVL1 antibody (16-418). Blue: DAPI for nuclear staining.