DDX6 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-16-517
Artikelname: DDX6 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-16-517
Hersteller Artikelnummer: 16-517
Alternativnummer: PRS-16-517-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human DDX6 (NP_001244120.1).
Konjugation: Unconjugated
Alternative Synonym: DDX6, P54, RCK, HLR2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 54kDa
NCBI: 1656
UniProt: P26196
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DDX6 antibody (16-517) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (16-517) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.