DDX6 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DDX6 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-517
• Supplier Catalog Number: 16-517
• Alternative Catalog Number: PRS-16-517-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human DDX6 (NP_001244120.1).
• Conjugation: Unconjugated
• Alternative Names: DDX6, P54, RCK, HLR2

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 54kDa
• NCBI: 1656
• UniProt: P26196
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using DDX6 antibody (16-517) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (16-517) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.