FGD1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-16-578
Artikelname: FGD1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-16-578
Hersteller Artikelnummer: 16-578
Alternativnummer: PRS-16-578-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2).
Konjugation: Unconjugated
Alternative Synonym: FGD1, AAS, FGDY, MRXS16, ZFYVE3
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 110kDa
NCBI: 2245
UniProt: P98174
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using FGD1 antibody (16-578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat kidney using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using FGD1 Polyclonal Antibody (16-578) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.