FGD1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-16-578
Article Name: FGD1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-16-578
Supplier Catalog Number: 16-578
Alternative Catalog Number: PRS-16-578-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2).
Conjugation: Unconjugated
Alternative Names: FGD1, AAS, FGDY, MRXS16, ZFYVE3
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 110kDa
NCBI: 2245
UniProt: P98174
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using FGD1 antibody (16-578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat kidney using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using FGD1 Polyclonal Antibody (16-578) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.