ERK1 / ERK2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ERK1 / ERK2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-16-712
• Hersteller Artikelnummer: 16-712
• Alternativnummer: PRS-16-712-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant Protein of human ERK1 / ERK2
• Konjugation: Unconjugated
• Alternative Synonym: ERK, ERT1, MAPK 1, MAPK2, ERK2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 42kDa/44kDa
• NCBI: 5594
• UniProt: P28482
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (16-712) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunofluorescence analysis of C6 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.