ERK1 / ERK2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ERK1 / ERK2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-712
• Supplier Catalog Number: 16-712
• Alternative Catalog Number: PRS-16-712-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant Protein of human ERK1 / ERK2
• Conjugation: Unconjugated
• Alternative Names: ERK, ERT1, MAPK 1, MAPK2, ERK2

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 42kDa/44kDa
• NCBI: 5594
• UniProt: P28482
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (16-712) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunofluorescence analysis of C6 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.