Western blot analysis of extracts of various cell lines, using COL1A1 antibody (16-789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry of paraffin-embedded human liver cancer using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Rat heart using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of mouse kidney using COL1A1 antibody (16-789) at dilution of 1:100. Blue: DAPI for nuclear staining.
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