COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-789
• Supplier Catalog Number: 16-789
• Alternative Catalog Number: PRS-16-789-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide of human COL1A1.
• Conjugation: Unconjugated
• Alternative Names: Alpha-1 type I collagenOI4

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 139kDa
• NCBI: 1277
• UniProt: P02452
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using COL1A1 antibody (16-789) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

Immunohistochemistry of paraffin-embedded human liver cancer using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded Rat heart using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using COL1A1 antibody (16-789) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of mouse kidney using COL1A1 antibody (16-789) at dilution of 1:100. Blue: DAPI for nuclear staining.