TOM20 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: TOM20 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-16-790
• Hersteller Artikelnummer: 16-790
• Alternativnummer: PRS-16-790-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein of human TOM20
• Konjugation: Unconjugated
• Alternative Synonym: MAS2MOM19, TOM2mitochondrial import receptor subunit TOM20 homolog, mitochondrial 20 kDa outer membrane protein, outer mitochondrial membrane receptor Tom2translocase of outer mitochondrial membrane 20 homolog type II

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 16kDa
• NCBI: 9804
• UniProt: Q15388
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using TOM20 antibody (16-790) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.

Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using TOM20 antibody (16-790) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 using TOM20 antibody (16-790) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using TOM20 antibody (16-790) at dilution of 1:100. Blue: DAPI for nuclear staining.