TOM20 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: TOM20 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-790
• Supplier Catalog Number: 16-790
• Alternative Catalog Number: PRS-16-790-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein of human TOM20
• Conjugation: Unconjugated
• Alternative Names: MAS2MOM19, TOM2mitochondrial import receptor subunit TOM20 homolog, mitochondrial 20 kDa outer membrane protein, outer mitochondrial membrane receptor Tom2translocase of outer mitochondrial membrane 20 homolog type II

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 16kDa
• NCBI: 9804
• UniProt: Q15388
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using TOM20 antibody (16-790) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.

Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using TOM20 antibody (16-790) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using TOM20 antibody (16-790) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 using TOM20 antibody (16-790) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using TOM20 antibody (16-790) at dilution of 1:100. Blue: DAPI for nuclear staining.