DNMT1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DNMT1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-16-953
• Hersteller Artikelnummer: 16-953
• Alternativnummer: PRS-16-953-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant fusion protein of Human DNMT1.
• Konjugation: Unconjugated
• Alternative Synonym: DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1, AIM

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 200kDa
• NCBI: 1786
• UniProt: P26358
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using DNMT1 antibody (16-953) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (16-953) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (16-953) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using DNMT1 antibody (16-953) at dilution of 1:100. Blue: DAPI for nuclear staining.