DNMT1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DNMT1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-16-953
• Supplier Catalog Number: 16-953
• Alternative Catalog Number: PRS-16-953-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein of Human DNMT1.
• Conjugation: Unconjugated
• Alternative Names: DNMT1, DNA (cytosine-5-)-methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1, AIM

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 200kDa
• NCBI: 1786
• UniProt: P26358
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using DNMT1 antibody (16-953) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using DNMT1 antibody (16-953) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (16-953) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DNMT1 antibody (16-953) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using DNMT1 antibody (16-953) at dilution of 1:100. Blue: DAPI for nuclear staining.