MonoMethyl-Histone H3-K27 pAb, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MonoMethyl-Histone H3-K27 pAb, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-624
• Hersteller Artikelnummer: 18-624
• Alternativnummer: PRS-18-624-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Other, Rat
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
• Konjugation: Unconjugated
• Alternative Synonym: H3F3A, H3t, H3.4, H3/g, H3FT

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 18kDa
• NCBI: 8290
• UniProt: Q16695
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K27 antibody (18-624).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody (18-624).

Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid cancer using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K27 antibody (18-624). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using MonoMethyl-Histone H3-K27 antibody (18-624) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.