MonoMethyl-Histone H3-K27 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MonoMethyl-Histone H3-K27 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-624
• Supplier Catalog Number: 18-624
• Alternative Catalog Number: PRS-18-624-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Other, Rat
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
• Conjugation: Unconjugated
• Alternative Names: H3F3A, H3t, H3.4, H3/g, H3FT

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 18kDa
• NCBI: 8290
• UniProt: Q16695
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

Images

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K27 antibody (18-624).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody (18-624).

Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid cancer using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using MonoMethyl-Histone H3-K27 antibody (18-624) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K27 antibody (18-624). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using MonoMethyl-Histone H3-K27 antibody (18-624) and rabbit IgG. P1 and P2 were located on ANO2 gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.