Asymmetric DiMethyl-Histone H3-R17 pAb, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Asymmetric DiMethyl-Histone H3-R17 pAb, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-642
• Hersteller Artikelnummer: 18-642
• Alternativnummer: PRS-18-642-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat, Zebrafish
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
• Konjugation: Unconjugated
• Alternative Synonym: H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 17kDa
• NCBI: 8356
• UniProt: P68431
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642).

Immunohistochemistry of paraffin-embedded rat lung using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:100. Blue: DAPI for nuclear staining.