Asymmetric DiMethyl-Histone H3-R17 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Asymmetric DiMethyl-Histone H3-R17 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-642
• Supplier Catalog Number: 18-642
• Alternative Catalog Number: PRS-18-642-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
• Conjugation: Unconjugated
• Alternative Names: H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 17kDa
• NCBI: 8356
• UniProt: P68431
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642).

Immunohistochemistry of paraffin-embedded rat lung using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 antibody (18-642) at dilution of 1:100. Blue: DAPI for nuclear staining.