HMGB1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HMGB1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-706
• Hersteller Artikelnummer: 18-706
• Alternativnummer: PRS-18-706-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
• Konjugation: Unconjugated
• Alternative Synonym: HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 29kDa
• NCBI: 3146
• UniProt: P09429
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (18-706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (18-706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using HMGB1 antibody (18-706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.