HMGB1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HMGB1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-18-706
• Supplier Catalog Number: 18-706
• Alternative Catalog Number: PRS-18-706-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
• Conjugation: Unconjugated
• Alternative Names: HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 29kDa
• NCBI: 3146
• UniProt: P09429
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (18-706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (18-706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using HMGB1 antibody (18-706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.