IGF2BP3 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IGF2BP3 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-19-372
• Hersteller Artikelnummer: 19-372
• Alternativnummer: PRS-19-372-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
• Konjugation: Unconjugated
• Alternative Synonym: CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like growth factor 2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IGF2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, cancer/testis antigen 98

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 70kDa
• NCBI: 10643
• UniProt: O00425
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Bilder

Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (19-372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (19-372) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug IGF2BP3 antibody (19-372). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (19-372) at a dilition of 1:1000.