IGF2BP3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: IGF2BP3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-19-372
• Supplier Catalog Number: 19-372
• Alternative Catalog Number: PRS-19-372-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
• Conjugation: Unconjugated
• Alternative Names: CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like growth factor 2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IGF2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, cancer/testis antigen 98

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 70kDa
• NCBI: 10643
• UniProt: O00425
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Images

Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (19-372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (19-372) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug IGF2BP3 antibody (19-372). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (19-372) at a dilition of 1:1000.