PGRMC1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PGRMC1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-19-760
• Hersteller Artikelnummer: 19-760
• Alternativnummer: PRS-19-760-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 44-195 of human PGRMC1 (NP_006658.1).
• Konjugation: Unconjugated
• Alternative Synonym: PGRMC1, HPR6.6, MPR

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 24kDa
• NCBI: 10857
• UniProt: O00264
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using PGRMC1 antibody (19-760) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human vermiform appendix using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using PGRMC1 Polyclonal Antibody (19-760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using PGRMC1 Polyclonal Antibody (19-760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using PGRMC1 Polyclonal Antibody (19-760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.