PGRMC1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: PGRMC1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-19-760
• Supplier Catalog Number: 19-760
• Alternative Catalog Number: PRS-19-760-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 44-195 of human PGRMC1 (NP_006658.1).
• Conjugation: Unconjugated
• Alternative Names: PGRMC1, HPR6.6, MPR

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 24kDa
• NCBI: 10857
• UniProt: O00264
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using PGRMC1 antibody (19-760) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human vermiform appendix using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using PGRMC1 antibody (19-760) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using PGRMC1 Polyclonal Antibody (19-760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using PGRMC1 Polyclonal Antibody (19-760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using PGRMC1 Polyclonal Antibody (19-760) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.