COX IV Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: COX IV Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-22-315
• Hersteller Artikelnummer: 22-315
• Alternativnummer: PRS-22-315-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1).
• Konjugation: Unconjugated
• Alternative Synonym: COX4I1, COX4, COXIV, MGC72016, COX4-1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 17kDa
• NCBI: 1327
• UniProt: P13073
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using COX IV Antibody (22-315) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat spleen using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using COX IV antibody (22-315). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (22-315). Western blot was performed from the immunoprecipitate using COX IV antibody (22-315) at a dilition of 1:1000.