COX IV Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: COX IV Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-315
• Supplier Catalog Number: 22-315
• Alternative Catalog Number: PRS-22-315-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1).
• Conjugation: Unconjugated
• Alternative Names: COX4I1, COX4, COXIV, MGC72016, COX4-1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 17kDa
• NCBI: 1327
• UniProt: P13073
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using COX IV Antibody (22-315) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat spleen using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using COX IV antibody (22-315) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using COX IV antibody (22-315). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (22-315). Western blot was performed from the immunoprecipitate using COX IV antibody (22-315) at a dilition of 1:1000.