DNAJB6 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-660
Artikelname: DNAJB6 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-660
Hersteller Artikelnummer: 22-660
Alternativnummer: PRS-22-660-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
Konjugation: Unconjugated
Alternative Synonym: DNAJB6, DKFZp566D0824, DnaJ, FLJ42837, HHDJ1, HSJ-2, HSJ2, MGC1152, MGC117297, MRJ, MSJ-1, DJ4, LGMD1D, LGMD1E
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 40kDa
NCBI: 10049
UniProt: O75190
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (22-660) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (22-660). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.