DNAJB6 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DNAJB6 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-660
• Supplier Catalog Number: 22-660
• Alternative Catalog Number: PRS-22-660-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
• Conjugation: Unconjugated
• Alternative Names: DNAJB6, DKFZp566D0824, DnaJ, FLJ42837, HHDJ1, HSJ-2, HSJ2, MGC1152, MGC117297, MRJ, MSJ-1, DJ4, LGMD1D, LGMD1E

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 40kDa
• NCBI: 10049
• UniProt: O75190
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (22-660) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (22-660). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using DNAJB6 antibody (22-660) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.