ASPSCR1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ASPSCR1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-22-973
• Hersteller Artikelnummer: 22-973
• Alternativnummer: PRS-22-973-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).
• Konjugation: Unconjugated
• Alternative Synonym: Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, ASPSCR1, ASPL, RCC17, TUG, UBXD9, UBXN9

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 80kDa
• NCBI: 79058
• UniProt: Q9BZE9
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (22-973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody (22-973). Western blot was performed from the immunoprecipitate using ASPSCR1 antibody (22-973) at a dilition of 1:1000.