ASPSCR1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ASPSCR1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-973
• Supplier Catalog Number: 22-973
• Alternative Catalog Number: PRS-22-973-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).
• Conjugation: Unconjugated
• Alternative Names: Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, ASPSCR1, ASPL, RCC17, TUG, UBXD9, UBXN9

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 80kDa
• NCBI: 79058
• UniProt: Q9BZE9
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (22-973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody (22-973). Western blot was performed from the immunoprecipitate using ASPSCR1 antibody (22-973) at a dilition of 1:1000.