YBX1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: YBX1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-23-119
• Hersteller Artikelnummer: 23-119
• Alternativnummer: PRS-23-119-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
• Konjugation: Unconjugated
• Alternative Synonym: NSEP1, YB1, BP-8, CSDB, DBPB, YB-1, CSDA2, NSEP1, NSEP-1, MDR-NF1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 49kDa
• NCBI: 4904
• UniProt: P67809
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (23-119) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (23-119) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.