YBX1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: YBX1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-23-119
• Supplier Catalog Number: 23-119
• Alternative Catalog Number: PRS-23-119-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
• Conjugation: Unconjugated
• Alternative Names: NSEP1, YB1, BP-8, CSDB, DBPB, YB-1, CSDA2, NSEP1, NSEP-1, MDR-NF1

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 49kDa
• NCBI: 4904
• UniProt: P67809
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (23-119) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (23-119) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (23-119) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using YBX1 antibody (23-119) at dilution of 1:100. Blue: DAPI for nuclear staining.