POR Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: POR Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-23-429
• Hersteller Artikelnummer: 23-429
• Alternativnummer: PRS-23-429-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).
• Konjugation: Unconjugated
• Alternative Synonym: POR, CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 77kDa
• NCBI: 5447
• UniProt: P16435
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using POR antibody (23-429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (23-429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (23-429) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.