POR Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: POR Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-23-429
• Supplier Catalog Number: 23-429
• Alternative Catalog Number: PRS-23-429-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).
• Conjugation: Unconjugated
• Alternative Names: POR, CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 77kDa
• NCBI: 5447
• UniProt: P16435
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using POR antibody (23-429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (23-429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (23-429) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal Antibody (23-429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.