IL25 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-23-479
Artikelname: IL25 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-23-479
Hersteller Artikelnummer: 23-479
Alternativnummer: PRS-23-479-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).
Konjugation: Unconjugated
Alternative Synonym: IL25, interleukin 25, IL17E, interleukin 17E, interleukin-17E, interleukin-25
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 24kDa
NCBI: 64806
UniProt: Q9H293
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using IL25 antibody (23-479) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (23-479) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human lung cancer cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.