IL25 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: IL25 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-23-479
• Supplier Catalog Number: 23-479
• Alternative Catalog Number: PRS-23-479-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).
• Conjugation: Unconjugated
• Alternative Names: IL25, interleukin 25, IL17E, interleukin 17E, interleukin-17E, interleukin-25

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 24kDa
• NCBI: 64806
• UniProt: Q9H293
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using IL25 antibody (23-479) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (23-479) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of human lung cancer cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat bronchus cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.