NEFH Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: NEFH Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-23-587
• Hersteller Artikelnummer: 23-587
• Alternativnummer: PRS-23-587-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
• Konjugation: Unconjugated
• Alternative Synonym: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 240kDa
• NCBI: 4744
• UniProt: P12036
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of mouse brain, using NEFH antibody (23-587) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (23-587) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (23-587) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (23-587) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of mouse brain using NEFH antibody (23-587) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain using NEFH antibody (23-587) at dilution of 1:100. Blue: DAPI for nuclear staining.