NEFH Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: NEFH Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-23-587
• Supplier Catalog Number: 23-587
• Alternative Catalog Number: PRS-23-587-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
• Conjugation: Unconjugated
• Alternative Names: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 240kDa
• NCBI: 4744
• UniProt: P12036
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Images

Western blot analysis of extracts of mouse brain, using NEFH antibody (23-587) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (23-587) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (23-587) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (23-587) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of mouse brain using NEFH antibody (23-587) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain using NEFH antibody (23-587) at dilution of 1:100. Blue: DAPI for nuclear staining.