DDX50 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DDX50 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-23-692
• Hersteller Artikelnummer: 23-692
• Alternativnummer: PRS-23-692-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1).
• Konjugation: Unconjugated
• Alternative Synonym: DDX50, GU2, GUB, mcdrh, RH-II/GuB

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 105kDa
• NCBI: 79009
• UniProt: Q9BQ39
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using DDX50 antibody (23-692) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse testis using DDX50 antibody (23-692) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX50 antibody (23-692) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using DDX50 antibody (23-692) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using DDX50 antibody (23-692) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DDX50 antibody (23-692) at dilution of 1:100. Blue: DAPI for nuclear staining.