DDX50 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DDX50 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-23-692
• Supplier Catalog Number: 23-692
• Alternative Catalog Number: PRS-23-692-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1).
• Conjugation: Unconjugated
• Alternative Names: DDX50, GU2, GUB, mcdrh, RH-II/GuB

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 105kDa
• NCBI: 79009
• UniProt: Q9BQ39
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using DDX50 antibody (23-692) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse testis using DDX50 antibody (23-692) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX50 antibody (23-692) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using DDX50 antibody (23-692) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using DDX50 antibody (23-692) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DDX50 antibody (23-692) at dilution of 1:100. Blue: DAPI for nuclear staining.