PLK4 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-23-947
Artikelname: PLK4 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-23-947
Hersteller Artikelnummer: 23-947
Alternativnummer: PRS-23-947-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3).
Konjugation: Unconjugated
Alternative Synonym: PLK4, polo-like kinase 4 (Drosophila), STK18, serine/threonine kinase 18, SAK, Sak, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 115kDa
NCBI: 10733
UniProt: O00444
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using PLK4 antibody (23-947) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (23-947) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).