PLK4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: PLK4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-23-947
• Supplier Catalog Number: 23-947
• Alternative Catalog Number: PRS-23-947-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3).
• Conjugation: Unconjugated
• Alternative Names: PLK4, polo-like kinase 4 (Drosophila), STK18, serine/threonine kinase 18, SAK, Sak, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 115kDa
• NCBI: 10733
• UniProt: O00444
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using PLK4 antibody (23-947) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (23-947) at dilution of 1:100 (20x lens).

Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).