CD155 Antibody [3F1], Rabbit IgG, Unconjugated, Monoclonal

Produktübersicht

• Artikelname: CD155 Antibody [3F1], Rabbit IgG, Unconjugated, Monoclonal
• Artikelnummer: PRS-24-687
• Hersteller Artikelnummer: 24-687
• Alternativnummer: PRS-24-687-0.2
• Hersteller: ProSci
• Kategorie: Antikörper
• Applikation: ELISA, FC
• Spezies Reaktivität: Mouse
• Immunogen: This antibody was raised by immunising rats with recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.
• Konjugation: Unconjugated
• Alternative Synonym: TAGE 3F1, Poliovirus receptor, cellular receptor for poliovirus, Pvr, Tumor-associated antigen 1, Taa1, Tage4, HVED

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: batch dependent
• Klon-Bezeichnung: [3F1]
• NCBI: 52118
• UniProt: Q8K094
• Puffer: PBS with 0.02% Proclin 300.

Anwendungsbeschreibung

• Application Verdünnung: For research use only.

Bilder

Binding curve of anti-CD155 antibody 3F1 (24-687) to recombinant mouse CD155 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse CD155 Fc-Fusion Protein (Pr00231-1.9, Antibody) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 ng/ml was performed using 24-687. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.

Immunofluorescence staining of RAW 264.7 cells using anti-CD155 antibody (3F1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric r version of 3F1 (24-687) at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-687- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor 488 secondary antibody.

Western Blot using anti-CD155 antibody 3F1 ( Mouse thymus lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric r version of 3F1 (24-687) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.