Optimal dilutions for each application to be determined by the researcher
Anwendungsbeschreibung:
Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (Formalin-paraffin): 1-2 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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