TNF alpha Antibody [4C6-H8], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: TNF alpha Antibody [4C6-H8], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-33-216
• Supplier Catalog Number: 33-216
• Alternative Catalog Number: PRS-33-216-100
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC
• Species Reactivity: Canine, Feline, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: A hexadecapeptide corresponding to aa 115-130 of human TNF alpha, conjugated to thyroglobulin, was used as the immunogen for this TNF alpha antibody.
• Conjugation: Unconjugated
• Alternative Names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/mL
• Clone Designation: [4C6-H8]
• NCBI: 7124
• UniProt: P01375
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher
• Application Notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (Formalin-paraffin): 1-2 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.