E-Cadherin Antibody [CDH1/1525], Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: E-Cadherin Antibody [CDH1/1525], Unconjugated, Mouse, Monoclonal
• Artikelnummer: PRS-33-574
• Hersteller Artikelnummer: 33-574
• Alternativnummer: PRS-33-574-100
• Hersteller: ProSci
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
• Konjugation: Unconjugated
• Alternative Synonym: Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.2 mg/mL
• Klon-Bezeichnung: [CDH1/1525]
• NCBI: 999
• UniProt: P12830
• Puffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher
• Anwendungsbeschreibung: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the E-Cadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Bilder

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human placenta stained with E-Cadherin antibody (CDH1/1525).

Western blot analysis A) partial recombinant protein B) human stomach lysate using E-Cadherin antibody (CDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).