E-Cadherin Antibody [CDH1/1525], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: E-Cadherin Antibody [CDH1/1525], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-33-574
• Supplier Catalog Number: 33-574
• Alternative Catalog Number: PRS-33-574-100
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
• Conjugation: Unconjugated
• Alternative Names: Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/mL
• Clone Designation: [CDH1/1525]
• NCBI: 999
• UniProt: P12830
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher
• Application Notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the E-Cadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Images

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human placenta stained with E-Cadherin antibody (CDH1/1525).

Western blot analysis A) partial recombinant protein B) human stomach lysate using E-Cadherin antibody (CDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).